>>>>>
_____
Welcome,
----- 

And hopefully I can help educate yourself on some information I will be examining regarding preparation and use of plastic or glass petri dishes for bacterial and psilocybin mycelial examination. Without further adeu, 
>>>>>
_____
An Introduction - Petri Dish Prep: Cleaning, Sanitizing, Sterilizing, and to Re-Use or Not
-----
_____
Picking up from where we last left off: https://hypnochain.com/hive-163105/@trezzahn/an-introduction-to-spore-growth-and-reproduction-in-a-controlled-environment-p1-4-of-in-a-two-blog-series , introduced prior,  https://stemgeeks.net/hive-163521/@trezzahn/psilocybe-mycelial-and-their-colonization-on-agar-agar
it is important to remember that while most petri dishes come prepared for bacterial work, it is always a good standard to follow disinfection procedures when working with new dishes. 

One may think they can reuse contaminated plastic dishes, if proper chlorine bleach soaking and disinfection is completed prior this is very slightly plausible, but alas petri dishes are cheap and the resulting contamination that may occur is not worth the time nor effort. As seen below with a well-grown plastic petri dish growth with little to no contamination, 

![IMG_20201124_151511.jpg](https://images.hive.blog/DQmVX4JSYx47zdr9x6xy2mKFvGy1jhoLA91cb5jdmarS6Af/IMG_20201124_151511.jpg)

Comparitively to one that has been reused, 


![20201226_113651.jpg](https://images.hive.blog/DQmQPYaj6y8qk8bWGMVTma7QYRUd6dLS9jnsMYcFVgWwmF8/20201226_113651.jpg)

Signs of contamination are evident in multiple forms. 
_____
Cleaning, Sanitizing, then Sterilizing 
-----
Acquire one bowl for washing, one plastic container or small bucket for soaking, and a plastic container for sterilizing.   In the first bowl, mix disinfecting soap and warm water to scrub the petri dishes. This is done with a soft non-abrasive cloth and toothbrush for approximately 30 seconds.   
In the next, mix 1/8th cup of Clorox Bleach with 1 1/8th cups of warm water to soak the petri dishes. Lift the dish out of the soapy water and drop into the Clorox-water for two minutes.   

In the last, pour 30-100mL of Isopropyl 90-99% Rubbing or Disinfectant Alcohol. With sterile tongs, transfer the dish from the Clorox-water after two minutes to the last container for 3 seconds and quickly lift out of the alcohol.   

Place the dish face down in a plastic container with the top slightly open in a circulated room to dry condensation from the inside of the dish for two hours, then seal with parafilm or masking tape store for further use with Agar, to be explained next week here.  
_____
Before working with Agar, one should ensure decontamination of all surface surrounding is done. While air flow and circulation should already be consistent, one should disinfect the pouring table and anything surrounding thoroughly. 

If not done, consistent transfers, or wedging -as previously examined on hypnochain- will have to be done, as seen in the below layout:



![IMG_20201113_092136.jpg](https://images.hive.blog/DQmQ4cbbLvbc2dopJh9wfK8W8iu9Pc4wwhz3ijGnSwWqVKj/IMG_20201113_092136.jpg)


The individual should sanitize their self through shower and usage of lab coat or fresh, cling-free, non-shedding clothing. Safety glasses, hairnet, and latex or other gloves should be worn for the pouring of the dishes.   

In the case that a Sealed Air Box – a closed top containment for contaminant free and still air transfer – is not available for use, high sanitary measures should be followed to reduce contamination risk of dishes during pouring, as this is increased tenfold without protection from air contamination.  

Remember, the less air flow when working with the lid of a petri dish off when we pour Agar in the future for a medium such as so below, the better; 


![20201123_174444.jpg](https://images.hive.blog/DQmUW2eJWnwj6oCzxLzz66AMtDh5j6yPvxeFSHg99LWF585/20201123_174444.jpg)

_____
_____ 

Hopefully this was an educational introductory read for anyone interested in preparation of petri dishes and the proper technique for cleaning. 
_____
_____
# **Updated Links (for Ecency {+app} and PeakD)**
-----
## *Ecency*
_____
## Starting Out, and Psychoactive Research
_____
#### /@trezzahn/psychoactive-research
#### or
#### https://ecency.com/steemgeeks/@trezzahn/psychoactive-research

#### Medical Benefits - Writing *(Articulate)*
#### /@trezzahn/a-cross-examination-of-the
#### or
#### https://ecency.com/hive-125125/@trezzahn/a-cross-examination-of-the 

_____
### Outline Postings
#### HypnoChain Posting
#### /@trezzahn/general-hypnochain-outline-month-following
#### or
#### https://ecency.com/hive-163105/@trezzahn/general-hypnochain-outline-month-following

#### StemGeeks Posting
#### /@trezzahn/petri-dish-technique-and-methodology-month-following
#### or
#### https://ecency.com/hive-163521/@trezzahn/petri-dish-technique-and-methodology-month-following

_____
### Introduction to Psilocybin
#### HypnoChain Posting
#### /@trezzahn/3f3eyz-psilocybe-mycelial-and-their-colonization-on-agar-agar
#### or
#### https://ecency.com/foo/@trezzahn/3f3eyz-psilocybe-mycelial-and-their-colonization-on-agar-agar

#### StemGeeks Posting
#### /@trezzahn/psilocybe-mycelial-and-their-colonization-on-agar-agar
#### or
#### https://ecency.com/hive-163521/@trezzahn/psilocybe-mycelial-and-their-colonization-on-agar-agar

_____
### Section 1
-----
#### Part 1.1
#### /@trezzahn/an-introduction-to-spore-growth-and-reproduction-in-a-controlled-environment-p1-4-of-in-a-two-blog-series
#### or
#### https://ecency.com/hive-163105/@trezzahn/an-introduction-to-spore-growth-and-reproduction-in-a-controlled-environment-p1-4-of-in-a-two-blog-series

#### Part 1.2
#### /@trezzahn/an-introduction-to-petri-dish-preparation-for-use-with-growth-medium-day-two-p1-4-in-a-two-blog-series
#### or
#### https://ecency.com/hive-163521/@trezzahn/an-introduction-to-petri-dish-preparation-for-use-with-growth-medium-day-two-p1-4-in-a-two-blog-series

#### Part 2.1
#### /@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series
#### or
#### https://ecency.com/hive-163105/@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series

#### Part 2.2
#### /@trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series
#### or
#### https://ecency.com/hive-163521/@trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series

#### Part 3.1
#### /@trezzahn/liquid-culture-what-it-is-methodology-and-colonization-and-further-work
#### or
#### https://ecency.com/hive-163105/@trezzahn/liquid-culture-what-it-is-methodology-and-colonization-and-further-work

#### Part 3.2
#### /@trezzahn/mycelium-growth-preparation-agar-additionals-general-timeframes-and-further-work-the-fun-part
#### or
#### https://ecency.com/hive-163521/@trezzahn/mycelium-growth-preparation-agar-additionals-general-timeframes-and-further-work-the-fun-part

#### Part 4.1
#### /@trezzahn/mycelial-storage-syringes-cold-storing-and-other-methods-p4-4-of-a-two-blog-series
#### or
#### https://ecency.com/hive-163105/@trezzahn/mycelial-storage-syringes-cold-storing-and-other-methods-p4-4-of-a-two-blog-series

#### Part 4.2
#### /@trezzahn/agar-petri-dish-methodology-technique-and-where-we-re-off-to-next-p4-4-of-a-two-blog-series
#### or
#### https://ecency.com/hive-163521/@trezzahn/agar-petri-dish-methodology-technique-and-where-we-re-off-to-next-p4-4-of-a-two-blog-series

_____
### Section 2
-----
#### Part 1.1
#### /@trezzahn/mycelial-caking-and-growth-an-introduction-to-colonization-mediums-a-continuation-of-section-1-section-s-2-p1-1-4-in-a-two-blog
#### or
#### https://ecency.com/hive-163105/@trezzahn/mycelial-caking-and-growth-an-introduction-to-colonization-mediums-a-continuation-of-section-1-section-s-2-p1-1-4-in-a-two-blog

#### Part 1.2
#### /@trezzahn/brown-rice-flour-and-rye-grain-substrate-a-scientific-examination-of-its-utilization-as-a-medium-for-mycelial-growth-a
#### or
#### https://ecency.com/hive-163521/@trezzahn/brown-rice-flour-and-rye-grain-substrate-a-scientific-examination-of-its-utilization-as-a-medium-for-mycelial-growth-a

#### Part 2.1
#### /@trezzahn/mycelial-consolidation-and-colonization-of-a-substrate-medium-a-continuation-to-colonization-mediums-cont-section-1-section-s-2
#### or
#### https://ecency.com/hive-163105/@trezzahn/mycelial-consolidation-and-colonization-of-a-substrate-medium-a-continuation-to-colonization-mediums-cont-section-1-section-s-2

#### Part 2.2
#### /@trezzahn/shotgun-fruiting-chamber-s-construction-use-and-utilization-a-continuation-of-section-2-p2-2-4-in-a-two-blog-series
#### or
#### https://ecency.com/hive-163521/@trezzahn/shotgun-fruiting-chamber-s-construction-use-and-utilization-a-continuation-of-section-2-p2-2-4-in-a-two-blog-series

#### Part 3.1
#### /@trezzahn/birthing-of-consolidated-mycelium-cakes-pailocybin-growth-a-continuation-of-section-2-p3-4-in-a-two-blog-series
#### or
#### https://ecency.com/hive-163105/@trezzahn/birthing-of-consolidated-mycelium-cakes-pailocybin-growth-a-continuation-of-section-2-p3-4-in-a-two-blog-series

#### Part 3.2
#### /@trezzahn/fruiting-and-growth-of-birthed-mycelium-cake-psilocybin-growth-a-continuation-of-section-2-p3-4-in-a-two-blog-series
#### or
#### https://ecency.com/hive-163521/@trezzahn/fruiting-and-growth-of-birthed-mycelium-cake-psilocybin-growth-a-continuation-of-section-2-p3-4-in-a-two-blog-series

#### Part 4
#### /@trezzahn/harvest-and-flush-of-birthed-mycelium-cake-substrate-psilocybin-growth-a-conclusion-of-section-2-p4-4-in-a-two-blog-series
#### or
#### https://ecency.com/hive-163521/@trezzahn/harvest-and-flush-of-birthed-mycelium-cake-substrate-psilocybin-growth-a-conclusion-of-section-2-p4-4-in-a-two-blog-series
_____
_____
## *PeakD*
_____
### Starting Out
#### https://peakd.com/trezzahn/@trezzahn/starting-out-future-work

## Psychoactive Research
#### https://peakd.com/steemgeeks/@trezzahn/psychoactive-research

#### Medical Benefits - Writing *(Articulate)*
#### https://peakd.com/hive-125125/@trezzahn/a-cross-examination-of-the

_____
### Outline Postings
#### HypnoChain Posting
#### https://peakd.com/hive-163105/@trezzahn/general-hypnochain-outline-month-following

#### StemGeeks Posting
#### https://peakd.com/hive-163521/@trezzahn/petri-dish-technique-and-methodology-month-following

_____
### Introduction to Psilocybin
#### HypnoChain Posting
#### https://peakd.com/hive-163105/@trezzahn/3f3eyz-psilocybe-mycelial-and-their-colonization-on-agar-agar

#### StemGeeks Posting
#### https://peakd.com/hive-163521/@trezzahn/psilocybe-mycelial-and-their-colonization-on-agar-agar

_____
### Section 1
-----
#### Part 1.1
#### https://peakd.com/hive-163105/@trezzahn/an-introduction-to-spore-growth-and-reproduction-in-a-controlled-environment-p1-4-of-in-a-two-blog-series

#### Part 1.2
#### https://peakd.com/hive-163521/@trezzahn/an-introduction-to-petri-dish-preparation-for-use-with-growth-medium-day-two-p1-4-in-a-two-blog-series

#### Part 2.1
#### https://peakd.com/hive-163105/@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series

#### Part 2.2
#### https://peakd.com/hive-163521/@trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series

#### Part 3.1
#### https://peakd.com/hive-163105/@trezzahn/liquid-culture-what-it-is-methodology-and-colonization-and-further-work

#### Part 3.2
#### https://peakd.com/hive-163521/@trezzahn/mycelium-growth-preparation-agar-additionals-general-timeframes-and-further-work-the-fun-part

#### Part 4.1
#### https://peakd.com/hive-163105/@trezzahn/mycelial-storage-syringes-cold-storing-and-other-methods-p4-4-of-a-two-blog-series

#### Part 4.2
#### https://peakd.com/hive-163521/@trezzahn/agar-petri-dish-methodology-technique-and-where-we-re-off-to-next-p4-4-of-a-two-blog-series


_____
### Section 2
-----
#### Part 1.1
#### https://peakd.com/hive-163105/@trezzahn/mycelial-caking-and-growth-an-introduction-to-colonization-mediums-a-continuation-of-section-1-section-s-2-p1-1-4-in-a-two-blog

#### Part 1.2
#### https://peakd.com/hive-163521/@trezzahn/brown-rice-flour-and-rye-grain-substrate-a-scientific-examination-of-its-utilization-as-a-medium-for-mycelial-growth-a

#### Part 2.1
#### https://peakd.com/hive-163105/@trezzahn/mycelial-consolidation-and-colonization-of-a-substrate-medium-a-continuation-to-colonization-mediums-cont-section-1-section-s-2

#### Part 2.2
#### https://peakd.com/hive-163521/@trezzahn/shotgun-fruiting-chamber-s-construction-use-and-utilization-a-continuation-of-section-2-p2-2-4-in-a-two-blog-series

#### Part 3.1
#### https://peakd.com/hive-163105/@trezzahn/birthing-of-consolidated-mycelium-cakes-pailocybin-growth-a-continuation-of-section-2-p3-4-in-a-two-blog-series

#### Part 3.2
#### https://peakd.com/hive-163521/@trezzahn/fruiting-and-growth-of-birthed-mycelium-cake-psilocybin-growth-a-continuation-of-section-2-p3-4-in-a-two-blog-series

#### Part 4
#### https://peakd.com/hive-163521/@trezzahn/harvest-and-flush-of-birthed-mycelium-cake-substrate-psilocybin-growth-a-conclusion-of-section-2-p4-4-in-a-two-blog-series

_____
_____

You can follow me on my 

personal blog:
------ 

https://hive.blog/@trezzahn 

and research blog: 
----- 

https://ecency.com/@trezzahn/blog
_____ 
-----
PeakD:
-----
https://peakd.com/@trezzahn 


hive.buzz:
-----
https://d.buzz/#/@trezzahn/t/buzz?ref=nav


_____
>>>>>
*Thanks again all for hopefully some potential interest, and look forward for more to come!*
-----
_____ 

Best regards, 

- Trezzahn
Psychoactive Research: https://hypnochain.com/steemgeeks/@trezzahn/psychoactive-research

_____
_____
*Old Links*
-----
_____

The examination of liquid cultures from growth of mycelium on HypnoChaiin Monday is inspected here:. https://hypnochain.com/hive-163105/@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series  ,and the preparing for use of these dishes with will be examined at the end of the week on StemGeeks, here:  https://stemgeeks.net/hive-163521/@trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series
_____
Yesterdays intro to growth can be found here: 

https://hypnochain.com/hive-163105/@trezzahn/an-introduction-to-spore-growth-and-reproduction-in-a-controlled-environment-p1-4-of-in-a-two-blog-series 

Or

https://stemgeeks.net/hive-163105/@trezzahn/an-introduction-to-spore-growth-and-reproduction-in-a-controlled-environment-p1-4-of-in-a-two-blog-series

Stemgeeks @ 
-----
https://stemgeeks.net/@trezzahn 

https://stemgeeks.net/hive-163521/@trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series

and the following week, the preparation of Agar Agar on StemGeeks (above), and mycelial utilization in liquids for culturing again on the HypnoChain blog (below), 

https://hypnochain.com/hive-163521/@trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series

And

https://stemgeeks.net/hive-163105/@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series

Or 

https://hypnochain.com/hive-163105/@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series

As well as this following weeks posting on Liquid Culture:

https://hypnochain.com/hive-163105/@trezzahn/liquid-culture-what-it-is-methodology-and-colonization-and-further-work

Or here,

https://stemgeeks.net/hive-163105/@trezzahn/liquid-culture-what-it-is-methodology-and-colonization-and-further-work

Hypnochain @ 
------
https://hypnochain.com/@trezzahn
_____
For full blog updates and for other communities, check out 

Ecency at: 
-----
https://ecency.com/@trezzahn/blog 

_____
_____ 

#mushroom #psilocybin #psilocybe #mycelium #mycelial #petri #spore #psychedelic #psychoactive #hypnotic #hive #ecency #hypnochain #stem #stemgeeks #proofofbrain

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<center>Posted on [HypnoChain](https://hypnochain.com/@trezzahn/an-introduction-to-petri-dish-preparation-for-use-with-growth-medium-day-two-p1-4-in-a-two-blog-series)</center>

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