Is the prevalent human prion protein 129M/V mutation a living fossil from a Paleolithic panzootic superprion pandemic?

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7030913/

Thiamin and Protein Folding
Dr Lonsdale

https://www.sciencedirect.com/science/article/abs/pii/S0306987719300829?via%3Dihub

Derrick Lonsdale
Publications

https://pubmed.ncbi.nlm.nih.gov/?term=Derrick%20Lonsdale

COVID-19 and Thiamine: An Interview with Dr. Derrick Lonsdale

https://www.hormonesmatter.com/covid-19-thiamine-interview-with-dr-derrick-lonsdale/

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Adenine–Thymine

Thymine
Thiamine

Pithovirus 
Pandoravirus

Thiamine Pyrophosphate
TPP Riboswitch

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Thirty-thousand-year-old distant relative of giant icosahedral DNA viruses with a pandoravirus morphology

https://www.pnas.org/doi/10.1073/pnas.1320670111

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https://www.askdifference.com/thymine-vs-thiamine/

Thiamin (Vitamin B1) Biosynthesis and Regulation: A Rich Source of Antimicrobial Drug Targets?

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020362/

The Structural and Biochemical Foundations of Thiamin Biosynthesis

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078420/

TPP Riboswitch

https://en.m.wikipedia.org/wiki/TPP_riboswitch

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Thiamine derivatives bind messenger RNAs directly to regulate bacterial gene expression

mRNAs encoding enzymes involved in thiamine (vitamin B1) biosynthesis in Escherichia coli can bind thiamine or its pyrophosphate derivative without the need for protein cofactors. The mRNA–effector complex adopts a distinct structure that sequesters the ribosome-binding site and leads to a reduction in gene expression. This metabolite-sensing regulatory system provides an example of a ‘riboswitch’ whose evolutionary origin might pre-date the emergence of proteins.

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the newest zombie virus from Siberia permafrost is directly linked to B1 metabolism.

it just seems odd that deadly viruses have a B1 Thiamine pathway, and B1 Thiamine is dependent upon Magnesium, and without this nutrient combo it goes into Adenine–Thymine and starts wrecking havoc on the DNA.

suspect mycoplasma incognitus, long covid, are all operating with some kind of slow acting Prion process.

Prion hides in bone marrow, same with mycoplasma.

also suspect a high dose of B1 Thiamine may be both protect DNA & mutate the "virus" to become non pathogenic, or part of the immunity, or beneficial.

i think the idea is, pathogenic virus/prions can be trained to leave DNA alone, by slamming B1 into the system, meaning any new variations of the same kind of virus will be preprogrammed to follow suit, and stay benign, essentially immunity.

it cost a billion dollers to make virus capable of converting into prion via GoF, and it cost 20 cents to reverse all of it, because B1 is technically an alkaloid, it can reset microbial traits.

have a few ideas regarding B1 Thiamine, the way high dose feels, what it may possibly be doing.

it reminds me of Menthol (Mint) or Vapor Rub (Hot&Cold).

Nitrogen (cold) & Sulfur (hot) bound to a 5-Carbon  Heterocycle, it goes into two opposite pathway reactions, acting as an Enzyme Activator.

also its identical to an Alkaloid, meaning it will help mitochondria, and mutate microbes.

apparently all the different pathogenic pathogens use B1 pathways, so many medications use B1 blockers, by High Dose B1 is flooding the microbes with too much, and binding them up, and mutates.

my suspension is this kind of "Over Stimulation" of a potential Alkaloid will not only bind up, cap off, and degenerate via enzymes, but also altering the pathogenic properties out of the pathogens, making them a positive component to the immune system, preventing other dangerous varieties.

conclusion is both Mycoplasma & Prion reinfection is absolutely certain, so the pathogens nature must be changed, and become part of the immune system.

the idea is to overwhelm the pathogens and convert them into beneficial microbes.

the old man who discovered B1 mega dose, was specifically because of a child with a deadly chromosome disease.

how does Spike not effect very specific genetics (gene pool) if its a Prion?

is it because this very specific gene pool has evolved with a form of Kuru, mislabeled genetic disorders, or disease?

if so, then the old man was on to something nobody can see, unfortunately at that time he probably didnt realize the importance of coenzyme with Magnesium, because eventually the child passed away, apparently by some kind of separate infection, meaning its possible the child immunity was compromised by Thiamine chelation of Magnesium, but at least the data was recorded.

beginning to suspect its only B1 capable of changing course of a superprion event, if the spike is actually a prion, reinfection is certain, and over time everything will have it, food water, plants animals.

B1 is very similar to Alkaloid & Heterocycle, meaning it can possibly not only bind the prion, but change it from pathogenic into probiotic, to protect as an immunity, apposed to autoimmunity.

just a hunch, but in my imagination, its B1 that can change the bioweapon into a immune protective.

many antiparasitic, antivirals are B1 blockers, meaning they use B1, my hunch is by doing the opposite will change the DNA program, to avoid B1, and the Alkaloid nature of it will make it permanent.

whats interesting is B1 is nontoxic, and binds to the most toxic elements prion.

its the opposite elemental compound.

all parasites seem to target Thiamine.

so by knocking out the parasitic elements, the microbe is not likely to revert back into pathogenic.

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B1 Thiamine:
Pyrimidine
Thiazole

by looking at the chemical compound, it looks like a few different hacks could be figured out.

Niacin (B3), MSM (DMSO) & Absorbic Acid (Vitamin C).

Vitamin C could probably be replaced with Citric or Vinegar.

Nitrogen & Sulfur could possibly be substituted with regular Fertilizer ingredients.

just thinking out loud, but it looks like all you need is a 80% (weak) Carbon acid, 5% Nitrogen & 5% Sulfur, and reduced down to the most simple Carbon form of 5‐6, meaning its very small.

if B1 has such powerful properties, then a full spectrum analysis, match the frequency, mimic the atomic code, by testing the compound directly, bypassing guess work, harmonic resonance spectrometry.

B1 seems to behave like an antioxidant, Oxythiamine being the oppsite, its almost like something needs to be flipped the correct direction, or spin.

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Human Virus Genomes Are Enriched in Conserved Adenine/Thymine/Uracil Multiple Tracts That Pause Polymerase Progression

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198555/

virus genomes present conserved multiple A (and T or U) tracts that could in principle form quadruplex structures. We proved that this is not the case, but these tracts are biologically active, as they trigger pausing of polymerase.

The prion protein binds thiamine

https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2011.08304.x

Using a combination of 1D NMR, fluorescence quenching and surface plasmon resonance, we found that thiamine (vitamin B1) was the only ligand that exhibited clear and specific binding to PrPc (Prion).

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Thiamine
Vascular

THTR1 (transporter)
SLC19A2 (gene)
ADRB1 (receptor)

TC1 (THTR1)
Thiamine Transporter 1

https://en.m.wikipedia.org/wiki/Thiamine_transporter_1

ADRB1
Beta-1 Adrenergic Receptor

https://en.m.wikipedia.org/wiki/Beta-1_adrenergic_receptor

G-protein coupled receptor associated with the Gs heterotrimeric G-protein that is expressed predominantly in cardiac tissue. In addition to cardiac tissue, beta-1 adrenergic receptors are also expressed in the cerebral cortex.

Polymorphisms in ADRB-1

One of the single nucleotide polymorphisms (SNPs) in ADRB-1 is the change from a cytosine to a guanine, resulting in a protein switch from arginine (389R) to glycine (389G) at the 389 codon position.

Cytosine 
Cytokine 
Arginine 
Glycine 
Thymine 
Thiamine
Aldehyde

Methylation
Alkylation

The Curious Chemical Biology of Cytosine: Deamination, Methylation and Oxidation as Modulators of Genomic Potential

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3262930/

Evolutionary analysis indicates that DNA alkylation damage is a byproduct of cytosine DNA methyltransferase activity

https://www.nature.com/articles/s41588-018-0061-8

Review of the synthesis and biological activity of thiazoles

https://www.tandfonline.com/doi/full/10.1080/00397911.2020.1854787

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Folates carry a negative charge, and thiamine a positive charge, at physiological pH.

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Structural characterization of CA1462, the Candida albicans thiamine pyrophosphokinase

https://bmcstructbiol.biomedcentral.com/articles/10.1186/1472-6807-8-33

Magnesium impairs Candida albicans immune evasion by reduced hyphal damage, enhanced β-glucan exposure and altered vacuole homeostasis

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9282612/

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Candida is in direct competition for B1, and Magnesium disrupts its immune hiding ability.

had so many powerful & instant results using vinegar & minerals ions, might try that idea along with the Thiamine (Thiazole).

literally instant results, it was so fast, just missing something, but too much made the skin feel pickled.

(weak) Carbonic acids like vinegar (or citric), along with salts cause an Ionic Alkaline effect (not acidic), and B1 is Nitrogen/Sulfur & 5-Carbon similar to Alkaloid.

regarding things like Candida, it seems likely it was used in the laboratory to work with other pathogens, almost like an extension of a larger biome or symbiont, not just working together but actively transferring information as a single unit or amoeba.

Amoeba
Amoeboid
Amoebozoa
Slime Mold
Candida

i think the idea is to take the most common human microbes & enhance them into something more, so hiding in plain sight.

the direct pathway from gut microbiome to brain biome, this may be what was "modified"?

16S rRNA mentioned regarding the brain microbiome, is also used to identify Anthrax plasmids.

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Plasmid
Polymorphism
16S rRNA
Aminoglycoside
Methyltransferase

Is There a Brain Microbiome?

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165828/

16S rRNA

https://en.m.wikipedia.org/wiki/16S_ribosomal_RNA

In-Depth Analysis of Bacillus anthracis 16S rRNA Genes and Transcripts Reveals Intra- and Intergenomic Diversity and Facilitates Anthrax Detection

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788319/

Research Updates of Plasmid-Mediated Aminoglycoside Resistance 16S rRNA Methyltransferase

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311965/

Genomics of microbial plasmids: classification and identification based on replication and transfer systems and host taxonomy

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379921/

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why is Thiazole toxic, and Zinc Thiazole not?

https://pubchem.ncbi.nlm.nih.gov/compound/thiazole

https://pubchem.ncbi.nlm.nih.gov/compound/Zinc-thiazole

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Thiazolide
Niclosamide

Nitazoxanide: A first-in-class broad-spectrum antiviral agent

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113776/