Viewing a response to: @aboutcoolscience/mrna-modifications-to-overcome-stability-and-delivery-issues
Try Mg2+ ions to neutralize negative charge of mRNA. Most nucleic acids exist in vivo as Mg2+ complexes, not just regular phosphate salts. However, the natural Mg2+ complexes may be more susceptible to RNAases! Use RNAase inhibitors, consider substituting Mn2+, or Zn2+, or Co2+, instead of Mg2+?
| author | qiyi |
|---|---|
| permlink | re-aboutcoolscience-mrna-modifications-to-overcome-stability-and-delivery-issues-20180824t210711267z |
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Unfortunately, if you deliver mRNA with RNAse inhibitors the cells will be all messed up.
| author | aboutcoolscience |
|---|---|
| permlink | re-qiyi-re-aboutcoolscience-mrna-modifications-to-overcome-stability-and-delivery-issues-20180824t224025977z |
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Next thing you know, you'll be telling me we can't inject people with guanidinium thiocyanate to prevent RNA degradation either! ;)
| author | effofex |
|---|---|
| permlink | re-aboutcoolscience-re-qiyi-re-aboutcoolscience-mrna-modifications-to-overcome-stability-and-delivery-issues-20180826t172352000z |
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